A pronounced gag reflex (GR) is a problem for the acceptance and delivery of dental treatment for children. Despite a range of management strategies, lasers represent a quantum leap forward in the treatment of pediatric dental patients. The aim of this study was to evaluate the effect of low level laser stimulation (electro-acupuncture) in controlling the gag reflex of patients requiring upper alginate impression. Forty-five patients participated in the study, and were divided into three groups (Groups A, B, and C) of fifteen patients in each group. GR assessment was estimated by using the Gagging Severity Index (GSI). Group A underwent a red-light soft magnetic field laser stimulation (electroacupuncture) on conception vessel 24 (CV 24) for one minute (min). Group B underwent a combination of laser acupuncture of CV 24 and acupressure on pericardium 6 (PC 6). Group C, formed the placebo group. During laser acupuncture and acupressure, a second impression was taken and the Gagging Prevention Index (GPI) was evaluated. Both the GSI and the GPI were recorded at three different stages of the dental impression making procedure, stage I- an empty impression tray, stage II- with a loaded tray, stage- III ability to keep the impression in the mouth until the alginate sets. Statistical analysis was done using the SPSS version 21 software (SPSS Inc., Chicago IL). A significant decrease in GPI values as compared to GSI values, was observed after the laser acupuncture in Group A and B (p<0.05). The average improvement between the GSI and the GPI scores before and during laser acupuncture and acupressure in Group B was 53.6 %, in Group A was 34.2 % and in Group C was 2.81 %. When the mean values of GSI and GPI scores of the empty tray, loaded tray, alginate set were compared among the three groups A, B, C before and during laser acupuncture and acupressure, the results were statistically significant (p<0.05). The study concluded that both techniques, laser acupuncture on acupuncture points CV 24 and the combination of laser acupuncture on acupuncture points CV 24 and acupressure point PC 6 were effective methods in controlling the
gag reflex.