The aim of this study was to compare the amount of bacteria and fungi in the air during oral surgical procedures between operations using micromotor and operations without micromotor. Bacteria and fungi in the air were collected before and during tooth extraction (non-micromotor using operations) or impacted tooth removal (micromotor using operations) by placing culture plates at 4 positions including the patient’s head and feet, operator’s side, and assistant’s side. The colony forming units were counted after culture at 37oC for 24-48 hours. The results revealed non-significant increasing of bacteria and fungi during oral surgical procedure in both groups (Mann-Whitney U test). During tooth extraction, the number of bacteria increased at all positions except at the operator’s side. During impaction removal, the number of bacteria decreased at the patient’s feet position and increased at other positions. The number of fungi decreased at the assistant’s side during extraction and also at the operator’s side and patient’s feet during impacted tooth removal, while increased at other positions (Wilcoxon Signed-rank test). In conclusion, oral surgical procedures, with or without using of micromotor, caused dispersion of bacteria and fungi in the air.