The purpose of this study was to develop wax cubes having the desired physical properties, for evaluating the chewing ability. The manufacturing and evaluating process can be done in a short time. The developed wax cubes can be used to evaluate the chewing ability among various subject age groups. Four types of wax were blended until they achieved the desired physical properties to create 10 mm. x 10 mm. x 10 mm. wax cubes. Forty-five subjects (21 males and 24 females) with normal occlusion were classified into 3 groups (Group 1: 20-29 years old, Group 2: 30-39 years old, and Group 3: 40-49 years old). The wax cubes were disinfected and stored in an incubator (37oC) for 24 hours then soaked in a water bath (37oC) for 10 minutes prior to testing. Each subject was assigned to chew four pieces of the wax cubes, one by one for ten chewing strokes each using habitual chewing patterns. The chewed wax was captured and analyzed by the Image J program (NIH) which calculated the percent of well mixed color areas. The results showed the average percentage of chewing ability based on well mixed areas in Group 1, 2 and 3 were 25.3±4.4, 23.6±5.7, and 16.5±5.5 (mean±s.d.), respectively. One-way ANOVA showed the differences between Group 1 and 3, Group 2 and 3 were statistical significance (p < .05). However, no statistically significance (p > .05) was obtained between gender in each group when analyzed by Independent t-test. In conclusion, the wax cubes developed in this study using an uncomplicated manufacturing and evaluating process can be used to identify chewing ability among different groups. Also, this study indicates chewing ability and age of subjects are negatively correlated.