Efficacy of Royal Jelly Extract on Inhibition of Candida Albicans Adherence on Various Types of Denture Base Material
Acrylic resin, Candida albicans, Royal jelly, Tissue conditioner
Denture stomatitis is a common disease found in 60 percent of denture wearers. The causes are denture trauma and the high concentration of Candida albicans adherence on the inner surface of denture. Microbial adherence is the initial stage and the most important process, which causes the disease. The aim of this research was to study the efficacy of crude royal jelly extract on inhibition of Candida albicans adherence on various types of denture base material. The specimens of heat-cured acrylic resin, self-cured acrylic resin and tissue conditioners were placed in a various concentration of crude royal jelly extract solution, using Sabouraud Dextrose Broth as a negative control group and Nystatin as a positive control group. The standard cell suspension was added in each well and incubated at 37ºC for 24 hours. The adherence of Candida albicans was determined using MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and SEM (scanning electron microscopy). Adherence of Candida albicans was found on both heat-cured and self-cured denture base acrylic for the negative control group, but was less found on both types of tissue conditioners. In addition, crude royal jelly extract solution at a concentration of 50 mg/mL and 25 mg/mL could significantly inhibited the adherence of Candida albicans when compare with the negative controls group (P < 0.05). The increase of royal jelly concentration further reduced the adherence of Candida albicans on both types of denture acrylic, which was consistent with the SEM result. There was no statistical significance (P > 0.05) between the type of acrylic resin and the adherence of Candida albicans. The results obtained from this research can be used as a baseline information for further development of royal jelly products as an antimicrobial agent especially for those who wear denture.