Salivary and Plaque Fluoride Level after MU Caries Preventive Program in Daycare Centers
Daycare center, Fluoride, MU caries preventive program, Plaque, Saliva
This study aimed to investigate and compare fluoride levels in saliva and plaque between the MU caries preventive program and a standard program. A randomized controlled trial was conducted on 77 preschool children from five daycare centers in Pathum Thani, Thailand. Children were randomly arranged into 2 groups: 1) a control group was provided a standard program including oral examination, oral hygiene instruction, diet advice and a fluoride varnish application; 2) a treatment group was provided the MU caries preventive program, which added extra interventions, including Interim Therapeutic Restoration (ITR) and sealant on posterior teeth with glass-ionomer cement. Plaque and saliva samples were collected before and after the program implementation at 24 hours, 1 week, 1 and 3 months, respectively. Salivary fluoride level was measured by a fluoride electrode, while plaque fluoride level was analysed by micro-diffusion technique and using a fluoride electrode (Model 96-09 Orion). The difference of plaque and salivary fluoride levels between the two groups was analyzed by Repeated ANOVA and Mann-Whitney U test, respectively. The treatment group showed a significantly higher plaque fluoride level than the control group at 24 hours (p<0.001), 1 week (p=0.018), and 1 month. (p=0.022). However, no significant difference was observed between the two groups at 3 months (p=0.228). The salivary fluoride levels showed the same tendency. The treatment group showed significantly higher salivary fluoride levels than the control group at 24 hours (p<0.001), 1 week (p<0.001), and 1 month (p=0.028). However, no significant difference was observed between the two groups at 3 months (p=0.055). This study was concluded that the plaque and salivary fluoride levels of children in MU caries preventive program were significantly higher when compared with the standard program at 24 hours, 1 week and 1 month.