This study aimed to survey on tobacco use including knowledge, attitude and practice on tobacco control among Thai dental students in 2006. The data collection used anonymous self-administered questionnaire recommended by WHO as part of The Global Health Professional Survey project, were sent to all dental students from 8 public dental faculties. The response rate was 83% including 2,425 students. The findings indicated that 17% of dental students had ever smoked cigarettes. Three percent of the respondents were current cigarette smokers. Prevalences of smokers were 8% in males and 0.8% in females. Twenty nine percent of smokers wanted to quit smoking now. More than 90% of students had positive attitude towards tobacco control including banning of cigarette sales to adolescent under 18 years of age, banning of all tobacco advertising and banning of smoking in public places. Ninety percent of the respondents stated they have been educated on tobacco hazard. However, only 17% received a specific training on tobacco cessation techniques. Eighty four percent of students desired to be trained on tobacco cessation counseling technique. It was also found that 2% of dental students reported that they regularly consumed alcohol. In conclusion, prevalence of smokers was low in Thai dental students. A majority of students requested a tobacco cessation training program in dental schools.