This case report presents a successful interdisciplinary dental treatment to manage edentulous spaces in a skeletal class III case using camouflage orthodontic treatment with autotransplantation of a tooth with complete root
formation, by four specialties: prosthodontics, endodontics, oral surgery, and orthodontics. The treatment plan included extraction of the mandibular left first premolar and the maxillary right first premolar as well as autotransplantation of the maxillary right first premolar into the maxillary left premolar edentulous area. The keys to success of the autotransplantation were: (1) endodontic treatment of the donor tooth prior to orthodontic tooth alignment with round wire, (2) preapplication of orthodontic force before extraction, (3) preparation of the recipient socket guided by the periapical radiograph and study casts, (4) preservation of the donor tooth in its own bleeding socket during trying in, and (5) the use of a short-term suture splint. Comprehensive orthodontic treatment was completed, with favourable results. The treatment time was 38 months. The transplanted tooth was in excellent condition for a porcelain crown restoration, but the patient declined the treatment. After seven years of follow-up, the transplanted tooth was still in good condition with no signs of inflammation, or root resorption. This article thoroughly discusses the decision making for the treatment sequence and appropriate approaches from each specialist.

This study aimed to develop and test the oral health literacy measuring tool for patients in the Comprehensive Dental Clinic, Faculty of Dentistry, Chiang Mai University. It was divided into two phases. In the first phase, the oral
health literacy measuring tool for patients in the Comprehensive Dental Clinic was developed from the literature reviews. In the second phase, the created tool was tested for its quality in comparison with the existing tool, namely the oral health literacy measuring tool for adult patients in the Thai version by Supasin Deeraksa (ThREALD-30). The multi-stage sampling was undertaken to obtain 112 samples as maintenance patients, aged over 50 years, in the Comprehensive Dental Clinic, Faculty of Dentistry, Chiang Mai University. The samples were required to answer the questions contained in both tools at the same time, whereby this process was completed within 15 minutes. Data collection was conducted from January 2021 to December 2021. The measuring tool consisted of 32 items, covering all six components: access to dental service information, understanding, questioning, decision-making, oral behavioral change, and retelling others. The study results showed that the tool contained face validity and concurrent validity. The convergent validity with ThREALD-30 was r=0.22 (p=0.022), the concurrent validity occurring regarding the education level (p=0.047). In addition, the reliability of the whole measurement form showed Cronbach’s alpha coefficient equal to 0.96. In conclusion, the developed oral health literacy measuring tool can be further used to evaluate oral health literacy among patients for dental treatment.

This study purposed to evaluate the effect of a 21-day Chatbot on caregivers’ knowledge, attitude and
practices for children aged 0 to 5 years old, as well as the chatbot’s engagement and satisfaction. This study has
2 parts; first was a retrospective quantitative research evaluating caregivers’ knowledge, attitude and practices for
children aged 0 to 5 years old and engagement, utilizing data from a 21-day Chatbot initiative in 7 provinces southern
Thai regions program by the Faculty of Dentistry at Prince of Songkla University and National Health Security Office
(NHSO). The second part was a prospective qualitative research to evaluate user satisfaction and motivations for utilizing chatbot. Two trained and calibrated interviewers performed online in-depth interviews with individuals
who attended chatbot for 7, 14, and 21 days. Results indicated that after using a 21-day Chatbot program, a high
percentage of parents were confident in their ability to clean their children’s teeth and understood the reasons,
consequence, and methods for avoiding child dental caries. All 21 days of the program were attended by 57.9% of
the caregivers. Almost of caregivers satisfied with simple assess to participate, features, replies, infographics, videos,
knowledge provided. Additionally, the could applied provided knowledge for daily practices. Discontinuation of use was
based on their own schedules and the stability of the platform, which may error and did not deliver messages every day.

The aim of this study was (1) to identify sealant retention and caries on sealed teeth among primary school
students by follow-up time, (2) to compare sealant retention between mobile dental clinic and hospital clinic and (3)
to compare caries between sealed and non-sealed teeth. The searching was done from two data sources; journals
related to dental health from 1993-2022, citation and PubMed database. Among the total of 3,832 publications, 17
were selected according to the inclusion criteria. The result showed that the percentages of complete retention at
6-12, 13-24 and 25-60 months were 53.80, 32.84 and 24.43, respectively and the percentages of caries on sealed
teeth at 6-12, 18-36 and 60 months were 5.78, 20.09 and 26.66. Compared between hospital and mobile dental
clinics at 12 months, the odd ratio of complete retention was 1.79 times (95% CI = 1.18, 2.61). The odd ratio of
caries on teeth receiving sealant and those not receiving sealant at 24 months was 1.44 (95% CI = 0.81, 2.69). In
conclusion, the effectiveness of the school sealant in Thai was low, both of sealant retention and caries prevention.
Dental sealants provided in hospital clinic showed more complete retention than mobile clinic. Caries on sealed
teeth lower than non-sealed teeth. This study suggested measures to increase the effectiveness of dental sealants;
assessment of dental sealant and re-sealant in needed children i.e. high-risk caries or loss of sealant retention.
Strategies to enhance accessibility to dental services should be considered along with ensuring service quality.

The purpose of this study was to analyze the total provider cost of three oral health service delivery models.
Dental services were provided in sub-district health-promoting hospitals in three Contracted Units of Primary Care
(CUPs) by a fixation of dental nurses (Model 1), a rotation of dental personnel from the hospital once a week (Model 2),
or a combination of fixed dental nurses and rotation of dental personnel from the hospital once a month (Model
3). The design was a retrospective study. Population was all CUPs in the 11th health region of Thailand. Purposive
sampling of one CUP per model was carried out in accordance with the criteria, implementation of such models for
at least three fiscal years (Fiscal year 2018 - 2020) and high level of data collection cooperation. Total direct costs of
providers in fiscal year 2020, including capital costs, labor costs, and material costs, were collected by data collection
forms, which were tested for quality by the consensus agreement of the three experts. Data analysis consisted of total
provider cost analysis and sensitivity analysis using Microsoft Excel version 2016. The results showed that the total
provider cost per client and per visit for Model 1, Model 2, and Model 3 were 284, 188, and 319 baht per client and
171, 132, and 175 baht per visit, respectively. The results of a one-way sensitivity analysis of indirect cost percentage
and number of clients for the past three fiscal years present a similar trend as the base case. The lowest to highest
provider costs were as follows: Model 2, Model 1, and Model 3. In conclusion, A CUP that provided dental services
in sub-district health promoting hospitals by rotating dental personnel from the hospital once a week was the most
economically valuable of the three patterns.

Denture base fracture is often found as a primary clinical mode failure because of low flexural strength and
repetitive stress. The fabrication of new dentures incurs high expenses and is time-consuming. Purpose: The aim
of this study was to evaluate the flexural strength of auto-polymerized acrylic resins reinforced with 0.5, 1, 3, 10
weight % silane-treated nano-aluminum oxide particles. Seventy-two rectangular (ISO standard 20795-1) acrylic resins
were fabricated and divided into six groups (n=12). Two control groups were an unmodified heat-polymerized acrylic
resin group and auto-polymerized acrylic resin group. The others were experimental groups incorporated with 0.5, 1, 3
and 10 weight % silane-treated nano-aluminum oxide particles. The flexural strength was measured by a three-point
bending test. One-way ANOVA and Tukey Honestly Significant Difference (HSD) were used for statistical analysis. The
means flexural strength of silane-treated nano-alumina reinforced auto-polymerized groups (Groups SA0.5, SA1, SA3
and SA10) (136.63, 128.46, 125.61 and 121.53 MPa) were significantly improved compared to that of the control
self-cured group (Group SC) (111.49 MPa) (p<0.05). Group SA0.5 showed the highest mean flexural strength compared
to group SA10 and SC. Group SA0.5 also had the mean flexural strength almost as high as group HC. The flexural
strength of auto-polymerized acrylic resins with low weight percentages of silane-treated nano-alumina tended to be
higher than those with high weight percentages of silane-treated nano-alumina. Flexural strength of auto-polymerized
acrylic resins reinforced with nano-alumina at 0.5 weight percent was comparable to that of heat-cured acrylic resins.

Success in pulp revascularization after autotransplantation tends to happen in a tooth with an incomplete root formation, while a tooth with a complete root formation needs a root canal treatment. However, recent studies
showed that apicoectomy facilitated the repair and revascularization process with promising outcomes. This study aimed to compare the incidences of pulp necrosis and root resorption of autotransplanted teeth with a complete root formation which underwent apicoectomy and teeth with an incomplete root formation. Patients with a history of autotransplantation received clinical and radiographic follow-up examination. The autotransplanted teeth were divided into two groups, the incomplete root formation group and the extraoral apicoectomized complete root formation group. Pulp and periradicular outcomes (pulp healing, pulp necrosis and presence of root resorption) were determined with an additional of cone-beam computed tomography investigation. The incidence of each outcome and prognostic factors were statistically compared. The result showed that the incomplete root formation group presented 40 % (4 of 10) pulp necrosis and 10 % (1 of 10) root resorption, while the extraoral apicoectomized complete root formation group presented 77.8 % (7 of 9) pulp necrosis and 66.7 % (6 of 9) root resorption. The periradicular status between the two groups was significantly different. No prognostic factor was found to be related to pulp outcome, however apicoectomy and recipient socket were found to be related to the periradicular outcome. Autotransplanted teeth with complete root formation undergoing extraoral apicoectomy increased the risk of pulp necrosis and root resorption. A totally prepared recipient socket without remaining periodontal ligament was also found to be related to root resorption.

This study evaluated the prevalence of midpalatal suture maturation stages in 8 to 18 years old patients and the relationship between chronological age and the suture maturation stages in a Thai population. The cone-beam
computed tomography (CBCT) images of the midpalatal suture from 240 subjects (110 males, 130 females) aged 8 to 18 years were classified into five stages (A-E). The distribution of the maturation stages was determined according to chronological age and sex. Chi-square test was used to compare the prevalence of maturation stages between male and female subjects. Spearman’s rank correlation analysis was performed to investigate the relationship between the maturation stage and chronological age. The results showed that the fused stages (D-E) were not seen in the prepubertal age group (8-11 years old). In the postpubertal age group (12-18 years old), the prevalence of nonfused stages (A-C) and fused stages (D-E) was 82.3% and 17.7% respectively. Stage C was the most prevalent (62.6%) in the postpubertal age group. Female showed a significantly higher prevalence of fusion than male (χ2 = 5.434, p=0.02). The correlation coefficient between chronological age and the suture maturation was 0.325 (p<0.001). In conclusion, fusion was not observed in females under 12 years old and males under 14 years old. Thus, CBCT might be recommended to verify the suture status before performing maxillary expansion in female ≥12 years old and males ≥14 years old. Overall, chronological age had a weak positive correlation with the suture maturation.

The purpose of this study was to compare the skeletal and upper airway stability at 6 months post-surgical treatment between the surgery-early approach and the conventional orthognathic surgery in patients with skeletal
Class III malocclusion who underwent one-jaw bilateral sagittal split ramus osteotomy setback surgery. Thirty-five patients were included and allocated into two groups based on pre-surgical orthodontic treatment: surgery-early
group (n = 15) and conventional orthognathic surgery (n = 20). Lateral cephalometric radiographs were taken before surgery (T0), immediately after surgery (T1), and 6 months after surgery (T2). Independent t-test and Mann-Whitney U test were used to analyze the data between the two groups. Paired t-tests and Wilcoxon signed-rank tests were used to analyze the data in each group. At 6 months after surgery (T1-T2), forward, upward, and counterclockwise rotational movements of the mandible in both groups were observed with no statistically significant difference. Changes in upper airway dimensions, when compared between the pre-post surgical phase (T2-T0) revealed that the surgery-early group showed a statistically significant decrease (p<0.05) in the oropharynx, while the conventional orthognathic surgery group showed statistically significant decrease (p<0.05) in the oropharynx and hypopharynx. A comparison between the two groups at 6 months post-surgical treatment revealed no statistically significant difference. Dental movement in both groups had no statistically significant difference in either the vertical or anteroposterior movement. Compared with the conventional orthognathic surgery group, the surgery-early group showed an equal amount of mandibular movement and upper airway change at 6 months post-surgical treatment.

This article evaluated the surface roughness of heat-cured acrylic resin before and after immersion in 4 different household agent solutions and a commercial denture cleansing solution after simulated 6-month and
12- month durations. Seventy-two specimens were fabricated from heat-cured acrylic resin and were divided into 6 groups (n=12); namely 4 household agents (100% clear vinegar, 5% acetic acid 0.1%, and 0.5% Sodium hypochlorite), a commercial denture cleansing solution, Polident® (Block Drug Company Inc, Memphis, TN38113, USA.) and tap water. The acrylic resin specimens were immersed for 10 min/cycle, 5 times/day for 36 days representing 6-month of clinical service, and continued for another 36 days representing 12-month of clinical service. The surface roughness (Ra, nm) was measured before and after simulated immersion. The data were compared using repeated ANOVA and Tukey’s test. The mean difference in the Ra after the 6-month and 12-month immersions in the control group and the Polident®, 100% clear vinegar, and 5% acetic acid groups was not significantly different (P > 0.05). In contrast, the mean Ra in the 0.1% and 0.5% sodium hypochlorite groups was significantly higher (P < 0.05) after the 6-month immersion. However, the Ra increased with a diminishing value after the 12-month immersion. The Ra of the specimens immersed in 0.1% and 0.5% sodium hypochlorite was significantly increased after 6-month, which decreased by 12-month immersion. The Ra in the 100% clear vinegar and 5% acetic acid groups were not significantly different from that of the Polident® group. Therefore, 100% clear vinegar and 5% acetic acid, which are household agents, can be an alternative option for routine use. Further study should be performed to evaluate whether 0.1% and 0.5% sodium hypochlorite might be an alternative option for denture cleansing.

Untreated bruxism can cause pathologic consequences to the components of the masticatory system leading to unnecessary treatments that are often complicated and costly. However, the damage could be prevented if the
condition is diagnosed earlier. Bruxism patients with asymptomatic or mild jaw symptoms usually refused to admit that they grind their teeth. Therefore, a reasonably priced tool with high sensitivity that is comfortable to wear
would be beneficial for early screening or diagnosing sleep bruxism. The aim of this clinical study is to investigate Bruxcore Plate (Brux Checker®) accuracy to diagnose sleep bruxism in known cases. Forty-four sleep bruxism participants with clear evidence of bruxofacets on the intraoral appliance were enrolled. Results showed that Brux Checker® had a sensitivity of 84.1% after one night of application and a sensitivity of 100% on four consecutive nights. In conclusion, at least four nights of Brux Checker® wearing is recommended for sleep bruxism diagnosis.

The year 2021 marks the breakthrough of the COVID-19 mRNA vaccine as a new class of medicine. The same fundamentals of the mRNA-based vaccine could facilitate the development of mRNA-based regenerative therapy. Our research group is working on mRNA encoding growth factors for periodontal regeneration in patients with periodontitis. The objective of this study was to investigate protein expression after local administration of mRNA encoding plateletderived growth factor-BB (PDGF) encapsulated in lipid nanoparticles (PDGF mRNA) in ligature-induced rat periodontitis. 3-0 silk was placed around the maxillary left second molar for two weeks and then removed, while the maxillary right second molar was left non-ligated. A significant bone loss analyzed by a stereomicroscope and micro-computerized tomography and gingival bleeding at the ligature sites were observed as compared to the non-ligature sites. To evaluate transfection and protein translation, different doses of PDGF mRNA including low dose (3 μg), medium dose (10 μg), high dose (30 μg), and DPBS (control) and LNPs alone were injected into rat gingiva at palatal side. The translated PDGF protein production was assessed 24 hours after the injection using enzyme-linked immunosorbent assay (ELISA). High levels of PDGF production were detected at both ligature and non-ligature sites. The mean PDGF levels in mRNA treated groups ranged from 10,912.54±1,893.94 to 51,883.91±7,415.45 pg/mg protein, whereas levels in DPBS control and LNPs alone were negligible. PDGF protein expression showed a trend of dose response but the differences between doses were not significant. Clinical findings at injected sites showed no erythema or swelling. The histological findings showed no evidence of LNPs and other foreign substances of mRNA formulation remaining in the tissues. In conclusion, this study of 24 hour-local delivery of PDGF mRNA-LNPs into rat periodontitis results in highly translated PDGF protein without profound local inflammatory response. However, further studies into the in vivo kinetics and therapeutic efficacy of mRNA are required.

Chronic lymphocytic leukemia deletion 7 (CLLD7) and chromosome condensation 1-like (CHC1L) proteins are putative tumor suppressor proteins that have never been studied in oral potentially malignant disorders (OPMDs).
This study aimed to evaluate the expression of these two proteins in OPMDs which encompassed oral leukoplakia, oral lichen planus (OLP), and oral lichenoid lesions (OLL). The histopathological features of oral leukoplakia were
acanthosis with or without hyperkeratosis and mild to severe epithelial dysplasia. Therefore, five groups including acanthosis with or without hyperkeratosis, mild, moderate, and severe dysplasia and the last group OLP/OLL were subjected to immunohistochemistry using normal oral mucosa as a control. In each case, random areas were selected and photographed after immunohistochemistry, then at least 1000 cells were counted. For CLLD7 and CHC1L, nuclear, cytoplasmic, and/or membrane staining were considered positive. Positive cells at different locations were evaluated. SPSS version 18 was used to compare the variation of protein expression among groups with a statistical significance at p<0.05. CLLD7 and CHC1L proteins were expressed in all cases of NOM and OPMDs. Compared to the acanthosis group, nuclear staining of CLLD7 was significantly lower in the severe epithelial dysplasia and the OLP/OLL groups. Although increased cytoplasmic staining of CLLD7 was observed in all OPMDs groups compared to the NOM group, a statistically significant difference was observed between the mild and moderate epithelial dysplasia groups compared to the NOM group. Regarding CHC1L staining, the percentage of nuclear staining was reduced, whereas membrane staining was increased in all OPMD groups compared to the NOM group. However, a statistically significant difference was observed between the severe epithelial dysplasia and OPL/OLL groups compared to the NOM group. In conclusion, the altered subcellular localization of CLLD7 and CHC1L in OPMDs suggests that the expression of these putative tumor suppressor proteins might be dysregulated during the early malignant transformation processes of oral cancer.

Osteoradionecrosis of the jaw is an incurable condition. Management of complete healing of the necrotic bone and clinical full mucosal coverage is challenging. Therefore, the prevention of osteoradionecrosis of the jaw
is worth considering. There was some evidence of using laser therapy for preventing osteoradionecrosis of the jaw. The purpose of this systematic review was to evaluate the effectiveness of laser therapy in the prevention of
osteoradionecrosis of the jaw. A systematic review was conducted on published articles in databases of MEDLINE, Embase, Cochrane Library, Scopus, Google Scholar and Thai-Journal Citation Index Center to identify the eligible
studies to compare the effectiveness to prevent osteoradionecrosis of the jaw. The latest search date was 29 May 2022. The included studies were assessed with two independent reviewers by using the Cochrane Risk of Bias Tool for randomized controlled trials or the Joanna Briggs Institute critical appraisal for case reports. Then the data was extracted by using the Cochrane Handbook for Systematic Reviews of Interventions. The two reviewers were calibrated. The agreement of assessment between the reviewers was 90 %. There were 24 articles included by title and abstract. Five articles were discarded because of duplication. Of 19 articles, there were four studies; one randomized controlled trial and three case reports, that met the eligible criteria. The level of the bias was low risk. In conclusion, there was a possibility of using laser therapy immediately after extraction to prevent osteoradionecrosis of the jaw by gaining faster tissue coverage. The combinations of using laser therapies; photobiomodulation and photodynamic therapy with antibiotics or pentoxifylline and tocopherol or platelet-rich fibrin allowed favorable clinical outcomes in prevention of osteoradionecrosis of the jaw.

This research aims to analyze the digital color-coded values of clinical gingival color images and create a gingival color database using digital images to measure the clinical gingival color levels. Theories of RGB and HSB
color models were applied in the present study. The research model was cross-sectional descriptive. There were two research methods: 1) Digital gingival color code analysis and 2) Digital gingival color reliability analysis. In the
first part, photographs of the gingiva of 99 patients who were admitted to the dental hospital, Faculty of Dentistry, Naresuan University, were taken. Adobe Photoshop CS6 software was employed for digital color code analysis. The digital color codes of the gingiva collected from the 143 samples were then examined for their reliability. Data from the study exhibited the total numbers of digital areas from the gingival images were 3,700 areas, with slightly red being the majority, 35.51%, followed by pale pink, red, and bluish-red, for 27.46, 25.43, and 11.59%, respectively. The RGB and HSB color models revealed the frequency of one or two codes using the statistical mode. In contrast, the red code showing ten performed by the RGB model and more codes in the HSB model. The reliability analysis of digital gingiva was calculated with an alpha coefficient of 0.655, which is a moderate confidence level. The analysis of dental images in the present study shows the promising potential to create a database of gingival color. The digital image databases are created on the Windows operating system and Microsoft Access software that can display data such as frequency, percentage, gingival color code, and color samples. Performing the software, users can basically input data consisting of gingival images, color codes, filter inputs, and search the database of RGB and HSB color models.

Coronavirus disease 2019 (COVID-19) is an emerging disease that puts aerosolized dental treatments at a high risk of transmission; therefore, up-to-date knowledge of aerosol control plays an essential role in determining
standard regulation in dental practice. The objective of this study was to compare the efficiency in reducing the dispersion of aerosols by using various types of intraoral and extraoral suctions. The study was conducted in a
closed acrylic box. A high-speed handpiece (Airotor) was used to create aerosols. The intraoral and extraoral suctions were divided into six groups (saliva ejector, high-power suction tip, side-wing tip, dome- shaped tip, EasyPrep®, and Extraoral suction). The relative humidity in the box was monitored at 1, 5, and 10 mins with a hygrometer and was repeated three independent times. The videos were also recorded during the experiment. Results showed that the saliva ejector alone had the most aerosol diffusion outside the mouth. The mean of relative humidity was highest and was significantly higher than other groups using a saliva ejector in combination with other suctions. After 1 minute of the procedure, the mean relative humidity in the group using the saliva ejector plus extraoral suction was significantly lower than that of the group using the saliva ejector plus the high-power suction tip at P-value = 0.038. When the saliva ejector was used with the extraoral suction, the means relative humidity were not different between groups after 5-10 mins. In conclusion, the present study provided preliminary information for considering instruments as needed and the most effective one in reducing the dispersion of aerosols. The knowledge from this research could be used as a guideline to improve the workflow or regulation in dental practice for safety.